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. 2020 Jul 28;10:12550. doi: 10.1038/s41598-020-69314-2

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Scatter plot and regression line of IENFD vs CCM results using the area adjusted and randomized sampling method, grouped by method of analysis (A: manual, B: automated). The dots represent the mean values from the individual participants and the dashed lines indicate the regression line. The Pearson’s correlation coefficients were r = 0.12 (manual method) and r = 0.13 (automated method) for the CNFD values, r = 0.11 (manual method) and r = 0.10 (automatic method) for the CNFL values and r = 0.066 (manual method) and r = 0.038 (automated method) for the CNBD values.