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. 2020 Jul 28;10:12550. doi: 10.1038/s41598-020-69314-2

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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(A) Flowchart of the new randomized sampling method and area calculation. (B) Adjusted area calculation using the 2D nucleator by newCAST. Right images illustrate how to delineate the area in focus. The area_after is estimated as: a_after = π · I², where l is the length of a test ray from the central cross to the intersection. (C) Calculation of the adjusted CCM value. Blue lines are identified and traced nerve fibers and green dots are identified nerve fiber branches.