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. 2020 Jul 28;10:12592. doi: 10.1038/s41598-020-69558-y

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Age-dependent change of laminins. Immunofluorescence staining of laminin-α5 in 30s sun-protected Caucasian skin (A), 60s sun-protected Caucasian skin (B), 30s sun-exposed Caucasian skin (C) and 60s sun-exposed Caucasian skin (D) [numbers indicate the age range (decade) of the skin donors].Immunofluorescence staining of laminin-α3 in 30s sun-protected Caucasian skin (F), 60s sun-protected Caucasian skin (G), 30s sun-exposed Caucasian skin (H) and 60s sun-exposed Caucasian skin (I). Laminin α5′s signal intensity (E) and laminin α3′s signal intensity (J) were analyzed using WINROOF 2013 image analyzing software (Mitani, Fukui, Japan, https://www.mitani-visual.jp/products/image_analys_ismeasurement/winroof/). mRNA expression levels of LAMA5 (K), LAMB1 (L), LAMA3 (M) and LAMB3 (N) were analyzed by qPCR. Bars: 100 μm. Data are expressed as mean ± SD of three independent experiments from each of 5 donors in I–L. *p < 0.05.