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. 2020 Jul 28;10:12608. doi: 10.1038/s41598-020-69476-z

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© This is a U.S. Government work and not under copyright protection in the US; foreign copyright protection may apply 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Comparison of the ¹³C (¹H coupled and decoupled) α- and β-anomers of KDO monomer signals, to those of the K15 capsular polysaccharide signals for determination of the anomeric configuration of KDO in K15. (A) Schemes showing the H3a to C1 three-bond torsion in α- and β-KDO, respectively. (B, C) ¹³C ¹H -coupled (red) and ¹³C ¹H -decoupled spectra of KDO (B) and K15 (C), demonstrating that α-KDO is the K15 constituent. Figure 5A was created with PyMol 2.4 (www.schrodinger.com/pymol); the coordinates for α/β-KDO were obtained from CarbBuilder (www.organ.su.se/gw/doku.php?id=carbbuilder).