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. 2020 Jul 28;10:12608. doi: 10.1038/s41598-020-69476-z

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© This is a U.S. Government work and not under copyright protection in the US; foreign copyright protection may apply 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Genetic organization of E. coli K15 (F8316-14:O6:K15:H16) capsule gene cluster and its similarity to E. coli K5 (Group 2). Regions 1 and 3 (green) are involved in capsule transport across the inner membrane. Region 2 (yellow) is serotype specific, encoding proteins for capsule biosynthesis. ORFs in blue represent the second set of kpsS and kpsC genes that are of Group 3 origin. Red speckled ORFs are 5′ and 3′ regions of IS630 family transposase. A single point mutation to form a stop codon, resulted in the breakup of a single ORF into 2 fragments.