Table 3.

Amine substrate scope (10 mM scale) of wild-type TsRTA^a.

Substrate		Conversion (%)
		after 30 min	after 24 h
(R)-Methylbenzylamined		75 ± 1	96 ± 1
(S)-Methylbenzylamineb		<0.5	<0.5
(±)-Methylbenzylaminebc		82 ± 3	94 ± 1
L-Alanine		n.d.	n.d.
D-Alanine		29 ± 4	30 ± 4
(±)-Alaninec		30 ± 4	31 ± 4
β-Alanine		6 ± 5	12 ± 4
Isopropylamine		28 ± 4	88 ± 4
Cadaverine		23 ± 4	44 ± 3
o-Xylylenediamine		n.d.	7 ± 5
p-Nitrophenethylamine		23 ± 4	53 ± 6

^aUnless otherwise stated, 10 mM benzaldehyde was used as the carbonyl acceptor. Conversions are based on the formation of either acetophenone or benzylamine, as determined by HPLC and calculated from a calibration curve. All reactions were carried out in triplicate. Standard errors include the SE of the calibration curve. (Ellison and Williams, 2012; Theodorou et al., 2012).

^b10 mM pyruvate.

^c20 Mm.

“ < 0.5” = calculated conversion smaller than uncertainty from calibration curve. “n.d.” = no product detected.