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. 2020 Jul 22;10:1067. doi: 10.3389/fonc.2020.01067

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Copyright © 2020 Lv, Guo, Qu, Che, Li, Wang, Gong, Wu, Liu, Liu and Xu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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PD-L1 is targeted by miR-429 to mediate tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) sensitivity. (A) Binding of miR-429 and PD-L1 predicted from starBase. The position of the 3′-UTR binding targets of miR-429 and PD-L1 were predicted on TargetScan. (B) mRNA expressions of PD-L1 in SGC7901, BGC823, MKN45, and HGC27 cells via quantitative PCR (qPCR). (C) Western blot analysis of PD-L1 expressions in SGC7901, BGC823, MKN45, and HGC27 cells. (D) Expressions of PD-L1 in tumor and normal tissues analyzed using the TCGA database on the GEPIA2 website. Results are the mean ± SEM of triplicate samples from three independent experiments. *P < 0.05, **P < 0.01.