Skip to main content
. 2020 Jul 22;10:1067. doi: 10.3389/fonc.2020.01067

Figure 5.

Figure 5

Effects of PD-L1 on tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) sensitivity in gastric cancer (GCa) cells. (A) Cells were transfected with si-PD-L1 and PD-L1 expression was assessed by Western blot analysis. (B) Cells were transfected with si-PD-L1 and the number of apoptotic cells were assessed by terminal deoxynucleotidyl transferase UTP nick-end labeling (TUNEL) staining after TRAIL treatment (100 ng/ml). Scale bar, 25 μm. (C) Cells were transfected with mimic-429, mimic-control, empty vector (EV), and PD-L1-OE (PD-L1 cDNA) lacking the 3′-UTR to rescue PD-L1 expression. The PD-L1 levels were assessed by Western blot. (D) SGC7901 and BGC823 cells were co-transfected with mimic-429, mimic-control, EV, and PD-L1-OE (PD-L1 cDNA) without the 3′-UTR and the proportion of apoptotic cells were assessed by TUNEL staining after TRAIL treatment (100 ng/ml). Scale bar, 25 μm. (E) SGC7901 and BGC823 cells were transfected with si-PD-L1 and annexin V/propidium iodide (PI) stained to detect apoptotic cells by flow cytometry after TRAIL treatment (100 ng/ml). (F) SGC7901 and BGC823 cells were co-transfected with mimic-429, EV, and PD-L1-OE (PD-L1 cDNA) without the 3′-UTR and annexin V/PI stained to detect apoptosis by flow cytometry after TRAIL treatment (100 ng/ml). (G) SGC7901 and BGC823 cells were transfected with si-PD-L1 and the expression of cleaved caspase-3 was assessed via Western blot analysis after TRAIL treatment (100 ng/ml). (H) SGC7901 and BGC823 cells were co-transfected with mimic-429, EV, and PD-L1-OE (PD-L1 cDNA) lacking the 3'-UTR and the expression of cleaved caspase-3 was detected by Western blot after TRAIL (100 ng/ml) treatment. Results are the mean ± SEM of triplicate samples from three independent experiments. *P < 0.05.