FIG 4.

Defects in heme uptake and trafficking functions reduce the cytosolic heme pool. (A) Changes in eGFP/mKATE2 fluorescence ratios of the CnHS in the indicated strains grown in the presence or absence of hemin (100 μM) at 0, 60, and 120 min. The experiments were performed as for Fig. 1C. The data represent the averages from three independent experiments ± SEMs. Note that the differences in the ratios for the WT strain and the vps45 mutant at time zero are not statistically significant. (B) Spot assays of 10‐fold serial dilutions of the indicated strains on medium supplemented as shown. Cells were starved for iron for 48 h prior to spotting. The plates were incubated for 4 days at 30°C before being photographed. YNB-Li is low-iron YNB medium supplemented with bathophenanthroline disulfonate (150 μM), CPZ; chlorpromazine.