FIG 3.

Apo- and Holo-VqmA activities are enhanced in the absence of oxygen. (A) Western blot showing VqmA-FLAG abundance in ΔvqmA Δtdh V. cholerae carrying pvqmA-FLAG and treated as shown for 1 h. DPO was supplied at 25 μM. (B) Transcriptional activity for the pvqmR-lacZ reporter from the same samples presented in panel A. Data were normalized to the level of VqmA-FLAG in panel A. In panel B, data represent the average values from biological replicates (n = 3), and error bars represent SD. Statistical significance was calculated using a two-tailed Student t test. Asterisks are as follows: **** denotes P < 0.0001.