Badiee 2012.

Study characteristics
Patient Sampling	Serum BDG collected twice weekly in a prospective study
Patient characteristics and setting	Between November 2008 and November 2009, all pediatric hematology patients at risk for IFI were enrolled. Sixty‐two patients aged 1 to 14 years (median 9 years old) were analyzed. Ten of these patients had proven or probable IFI
Index tests	Glucatell test using 80 pg/mL as cut‐off for positivity
Target condition and reference standard(s)	Invasive aspergillosis determined by the 2008 EORTC criteria with BDG excluded
Flow and timing	Unclear time frame between sample and reference standard; all received index test and reference standard and were included in the analysis
Comparative
Notes
Methodological quality
Item	Authors' judgement	Risk of bias	Applicability concerns
DOMAIN 1: Patient Selection
Was a consecutive or random sample of patients enrolled?	Yes
Was a case‐control design avoided?	Yes
Did the study avoid inappropriate exclusions?	Yes
Could the selection of patients have introduced bias?		Low risk
Are there concerns that the included patients and setting do not match the review question?			Low concern
DOMAIN 3: Reference Standard
Is the reference standards likely to correctly classify the target condition?	Yes
Were the reference standard results interpreted without knowledge of the results of the index tests?	Unclear
Could the reference standard, its conduct, or its interpretation have introduced bias?		Low risk
Are there concerns that the target condition as defined by the reference standard does not match the question?			Low concern
DOMAIN 4: Flow and Timing
Was there an appropriate interval between index test and reference standard?	Unclear
Did all patients receive the same reference standard?	Yes
Were all patients included in the analysis?	Yes
Could the patient flow have introduced bias?		Low risk