Kami 2001.

Study characteristics
Patient Sampling	Both retrospective and prospective BDG samples were collected from patients
Patient characteristics and setting	All BMT patients were included in this study, as well as some high‐dose chemotherapy patients. Of the 122 patients, 33 had proven invasive aspergillosis. A majority (76%) were male, and patients ranged in age from 17 to 80 years
Index tests	Fungitec‐G test using 20 pg/mL as cut‐off for positivity
Target condition and reference standard(s)	Invasive aspergillosis diagnosed by histologic evidence and positive for Aspergillus in sputum, biopsy, or autopsy
Flow and timing	Unclear time frame between sample and reference standard; all received index test and reference standard and were included in the analysis
Comparative
Notes
Methodological quality
Item	Authors' judgement	Risk of bias	Applicability concerns
DOMAIN 1: Patient Selection
Was a consecutive or random sample of patients enrolled?	Yes
Was a case‐control design avoided?	No
Did the study avoid inappropriate exclusions?	No
Could the selection of patients have introduced bias?		High risk
Are there concerns that the included patients and setting do not match the review question?			High
DOMAIN 3: Reference Standard
Is the reference standards likely to correctly classify the target condition?	Yes
Were the reference standard results interpreted without knowledge of the results of the index tests?	Unclear
Could the reference standard, its conduct, or its interpretation have introduced bias?		Low risk
Are there concerns that the target condition as defined by the reference standard does not match the question?			Low concern
DOMAIN 4: Flow and Timing
Was there an appropriate interval between index test and reference standard?	Unclear
Did all patients receive the same reference standard?	Yes
Were all patients included in the analysis?	Yes
Could the patient flow have introduced bias?		Low risk