Senn 2008.

Study characteristics
Patient Sampling	Serum BDG collected twice weekly in the absence of fever, and daily if fever was present, as part of a prospective study
Patient characteristics and setting	95 adult hematologic patients hospitalized for myeloablative chemotherapy between 2002 and 2006. There were 30 cases of proven or probable IFI. The average age of patients was 57 years (range 19 to 77 years), and 61% were male
Index tests	Wako test using 11 pg/mL as cut‐off for positivity
Target condition and reference standard(s)	Mixed IFI as determined by the 2002 EORTC criteria
Flow and timing	Median time interval between sample and reference standard was 7.5 days, with a range of 0 to 51 days; all received index test and reference standard and were included in the analysis
Comparative
Notes
Methodological quality
Item	Authors' judgement	Risk of bias	Applicability concerns
DOMAIN 1: Patient Selection
Was a consecutive or random sample of patients enrolled?	Yes
Was a case‐control design avoided?	Yes
Did the study avoid inappropriate exclusions?	Yes
Could the selection of patients have introduced bias?		Low risk
Are there concerns that the included patients and setting do not match the review question?			Low concern
DOMAIN 3: Reference Standard
Is the reference standards likely to correctly classify the target condition?	Yes
Were the reference standard results interpreted without knowledge of the results of the index tests?	Unclear
Could the reference standard, its conduct, or its interpretation have introduced bias?		Low risk
Are there concerns that the target condition as defined by the reference standard does not match the question?			Low concern
DOMAIN 4: Flow and Timing
Was there an appropriate interval between index test and reference standard?	No
Did all patients receive the same reference standard?	Yes
Were all patients included in the analysis?	Yes
Could the patient flow have introduced bias?		High risk