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qRT-PCR analysis of Nestin mRNA expression in DPSCs post-transfection. As shown by the data, Nestin mRNA expression was upregulated 6 h post-transfection as compared with that of the control (Mann–Whitney, P = 0.004) and downregulated 24 h (Mann–Whitney, P = 0.029) and 48 h (Mann–Whitney, P = 0.004) post-transfection. No statistically significant alteration in Nestin mRNA expression was observed 16 h post-transfection. The data were normalized to expression levels in the control.
