Fig. 3.
Effects of SIKE on TBK1/NF-κB signaling pathway in PM2.5-treated cells. (A) L02 cells were infected with Ad-shRNA, Ad-shSIKE, Ad-GFP or Ad-SIKE for 24 h. Then, all cells were harvested for western blotting of SIKE expression levels. L02 cells were infected with Ad-shRNA, Ad-shSIKE, Ad-GFP or Ad-SIKE for 24 h, and were then subjected to PM2.5 (100 μg/ml) exposure for another 24 h. Subsequently, (B) RT-qPCR was used for IL-1β, IL-6, TNF-α and IFN-β mRNA levels. (C) Western blot analysis was performed for p-TBK1 and p-NF-κB protein expression levels in whole cells. (D) Western blot analysis of NF-κB in nuclear of cells as treated. (E) Immunofluorescence staining for NF-κB (green fluorescence) and p-TBK1 (red fluorescence) in cells. Scale bar was 25 μm. Data are expressed as means ± SEM (n = 3 independent observations). *P < 0.05 and **P < 0.01.