Fig. 4.
SIKE-regulated inflammatory response requires TBK1 blockage in PM2.5-treated cells. L02 cells were infected with Ad-GFP, Ad-SIKE, Ad-TBK1 or the combination of Ad-SIKE and Ad-TBK1 for 24 h, followed by PM2.5 (100 μg/ml) exposure for another 24 h. Then, (A) RT-qPCR was used to determine IL-1β, IL-6, TNF-α and IFN-β mRNA levels. (B) Western blot analysis was conducted for cellular p-NF-κB. L02 cells were infected with Ad-shRNA, Ad-shSIKE, Ad-shTBK1 or the combination of Ad-shSIKE and Ad-shTBK1 for 24 h, followed by PM2.5 (100 μg/ml) incubation for another 24 h. Next, all cells were harvested for further experiments. (C) RT-qPCR analysis of IL-1β, IL-6, TNF-α and IFN-β in cells. (D) Western blot analysis for p-NF-κB protein expression levels. Data are expressed as means ± SEM (n = 3 independent observations). *P < 0.05 and **P < 0.01.