Fig. 9.

Juglanin ameliorates PM_2.5-induced hepatic injury through improving Nrf2/SIKE signaling pathway in mice. (A) Body weight change of mice (n = 8 per group). (B) MBP results for mice (n = 8 per group). (C) Liver weight of mice (n = 8 per group). (D) Up panel, H&E staining for liver samples; down panel, immunofluorescence staining of Nrf2 (green) and SIKE (red) in liver sections (n = 5 per group). Scale bar was 50 μm. (E) Western blot analysis for liver Nrf2, SIKE, p-TBK1 and p-NF-κB (n = 5 per group). (F) RT-qPCR results for hepatic Nrf2 mRNA expression levels (n = 5 per group). (G) SOD and MDA in liver tissues were measured (n = 8 per group). (H) RT-qPCR results for hepatic IL-1β, IL-6, TNF-α, IFN-β, HO-1, NQO-1, GCLC and GCLM (n = 5 per group). Data are expressed as means ± SEM. *P < 0.05 and **P < 0.01; ns, no significant difference. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)