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. 2020 Jul 20;2020:5459209. doi: 10.1155/2020/5459209

Figure 2.

Figure 2

LPS-induced autophagy was mediated by TNF-α in THP-1 cells. (a) LPS-induced autophagy in a dose-dependent manner. THP-1 cells were treated with indicated concentration of LPS for 12 h. (b) THP-1 cells were treated with 1 μg/ml LPS for 6, 12, or 24 h. (c) Effect of specific anti-TNF-α monoclonal antibody infliximab on LPS-induced autophagy. THP-1 cells were preincubated with 5 μm infliximab for 1 h followed with 1 μg/ml LPS for 12 h. (d, e, and f) Densitometric analysis of the blots showing the ratios of LC3-II to LC3-I. Data were represented as the means ± S.D. (N = 4, ∗p < 0.05, ∗∗p < 0.01 vs. control; ▲p < 0.05 vs. LPS). IN: infliximab.