Skip to main content
. 2020 Jul 20;2020:5459209. doi: 10.1155/2020/5459209

Figure 5.

Figure 5

An intimate crosstalk between the NF-κB pathway and autophagy flux in LPS-stimulated THP-1 cells. (a) THP-1 cells were preincubated with 5 μm BAY11-7082 for 1 h followed by 1 μg/ml LPS for the indicated time. NF-κB and phospho-NF-κB were assessed by western blotting. (b) Expression of phospho-NF-κB relative to NF-κB. Data represented as the means ± S.D. (N = 4, ∗p < 0.05 vs. LPS 30′ or 60′). (c) BAY11-7082 significantly suppressed TNF-α production in LPS-stimulated THP-1 cells treated with minocycline. Data represented as the means ± S.D. (N = 3, ∗∗∗p < 0.001). (d) THP-1 cells were preincubated with 5 μm BAY11-7082 for 1 h followed by 1 μg/ml LPS with or without minocycline for 12 h. LC3s in cell lysate from different treatment groups were assessed by western blotting. (e) Expression of LC3-II relative to LC3-I. Data were represented as the means ± S.D. (N = 3, ∗p < 0.05 vs. LPS, ▲p < 0.05 vs. LPS+Mino). (f) THP-1 cells were preincubated with 5 μm BAY11-7082 or 20 μM CQ for 1 h followed with 1 μg/ml LPS for 12 h. NF-κB and LC3 were assessed by western blotting. (g) Expression of LC3-II relative to LC3-I and NF-κB relative to actin. Data were represented as the means ± S.D. (N = 3, ∗p < 0.05, ∗∗p < 0.01). BAY: BAY11-7082; Mino: minocycline; Ctrl: control; CQ: chloroquine.