Table 1.

Reagents, buffers, and solutions used in the SARS-CoV-2 in-cell S protein ELISA.

Reagent	Composition
Phosphate buffer	PBS
Fixation solution	8% (w/v) paraformaldehyde in PBS
Permeabilization solution	0.1% (v/v) Triton X-100 in PBS
Washing buffer	0.3% (v/v) Tween 20 in PBS
Antibody diluent	10% (v/v) FCS in washing buffer
First antibody	1:5,000 dilution of anti-SARS-CoV-2 S protein antibody 1A9 (Biozol GTX-GTX632604) in antibody diluent
Secondary antibody	1:15,000 dilution of HRP-conjugated anti-mouse antibody (Thermo Fisher #A16066) in antibody diluent
Peroxidase Substrate	3,3′,5,5′-tetramethylbenzidine (TMB)
Stop solution	0.5 M H2SO4