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. 2020 Jul 28;48(7):0300060520943792. doi: 10.1177/0300060520943792

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© The Author(s) 2020

Creative Commons Non Commercial CC BY-NC: This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (https://creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage).

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Figure 5. — BECN1 was a direct target of miR-28-5p. (a) Complementarity between miR-28-5p and the 3′-untranslated region (UTR) of BECN1. (b) The conserved binding sites in 3′-UTR of the BECN1 gene in various species. (c) Dual-luciferase reporter assay of the interaction between miR-28-5p and BECN1. (d) Protein expression of BECN1 in OCI-LY7 cells following transfection with an miR-28-5p mimic, miR-28-5p inhibitor, or negative control (NC). (e) The transfection efficiency was assessed by qRT-PCR. (f) The mRNA levels of BECN1 in OCI-LY7 cells. N = 6. Compared with the control group, *P < 0.05, **P < 0.01, ***P < 0.001. Cir, curcumin.