The
G6PD gene is located on the X chromosome, such that females have two genes and males have only one. Males with a mutated
G6PD allele (in red, G6PD
DEF) that expresses a compromised (deficient) G6PD enzyme protein typically have a blood G6PD value of less than 30% of normal. Females with two mutated G6PD-deficient alleles (in red, G6PD
DEF1, DEF2) also typically have a blood G6PD value of less than 30% of normal. Males with a wild type
G6PD allele (in green, G6PD
WT) that expresses a fully functional enzyme have G6PD activity in an approximate normal distribution around the 100% median, as do females with two wild type
G6PD alleles (in green, G6PD
WT1, WT2). Heterozygous females with both wild type and mutated
G6PD alleles (in yellow, G6PD
WT, DEF1) can express a spectrum of whole blood G6PD activity, ranging from severely deficient (<30%) to beyond the World Health Organization definition of normal for females (>80%), with the majority in the intermediate (30% to 80%) activity range. The colored zones indicate the distribution of enzymatic activities associated with the genotypes as described above; the blue line represents the cumulative G6PD activity-based histogram.