Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Jun 5;20(2):1307–1314. doi: 10.3892/etm.2020.8844

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright: © Zhang et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

PMC Copyright notice

3D culture of kidney organoids. (A) Bright field observations of the kidney organoids. (B) Hematoxylin and eosin staining results of the kidney organoids. (C) Expression detection of renal tubular markers LTL and ECAD in kidney organoids by immunofluorescence. (D) Expression detection of collection tube markers GATA3 and ECAD in kidney organoids by immunofluorescence. Scale bars, 100 µm. (E) Expression detection of kidney podocytes marker PODXL in kidney organoids by immunofluorescence. Scale bars, 50 µm. (F) Expression detection of the molecular markers of podocyte (NPHS1, PODXL and WT1) by western blotting. (G) Expression detection of the specific markers for ureteric buds RET and WNT11 by western blotting. The data are representative from a minimum of three independent experiments. LTL, Lotus tetragonolobus lectin; ECAD, e-cadherin; GATA3, GATA binding protein 3; NPHS1, nephrin; PODXL, podocalyxin; WT1, Wilms tumor protein; RET, proto-oncogene tyrosine-protein kinase receptor Ret; WNT11, protein Wnt-11; D, day.