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. Author manuscript; available in PMC: 2021 Mar 1.
Published in final edited form as: Arterioscler Thromb Vasc Biol. 2020 Jan 2;40(3):638–655. doi: 10.1161/ATVBAHA.119.313248

Figure 5.

Figure 5.

A. Alizarin Red S staining for calcium in 14 day VEC treated with GM, OGM, OGM+TNFα(30 ng/ml), or OGM+p65 over-expression. Scale bar is 100μm. B. Quantification of calcium deposition using ARS dye-extraction assay, normalized to GM + empty vector control (n = 6). (*: significant from groups 1, 2 #: significant from group 3, p < 0.05). C. αSMA protein expression in VEC with or without p65 over-expression with 14-day control or OGM treatment. Scale bar is 20μm. D. ACTA2 mRNA in VEC, same conditions as C (n = 4). E. Runx2 protein expression in VEC, same conditions as C. Scale bar is 20μm. F. Runx2 mRNA in VEC, same conditions as E (n = 4). G. Osteocalcin (OCN) protein expression in VEC, same conditions as C. H. Osteocalcin mRNA in VEC, same conditions as G. Statistical significance between different groups are indicated by unshared letter assignments, p < 0.05. Different letters indicate statistically significant differences between groups (p < 0.05). Error bars indicate SEM.