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. 2020 May 19;20(2):1169–1175. doi: 10.3892/etm.2020.8771

Figure 4.

Figure 4

miR-425 inhibits the proliferation of MEC-1 cells through regulation of the BTK/PLCγ2 signalling pathway. (A) mRNA and (B) protein expression levels of BTK, PLCγ2, Ki-67 and PCNA in cells transfected with miR-425 or NC at 48 h after transfection, as detected by reverse transcription-quantitative polymerase chain reaction and western blotting, respectively. (C) BTK protein expression levels in cells transfected with pcDNA3.1-BTK or pcDNA3.1-NC, and control cells, detected by western blotting at 48 h after transfection. (D) Proliferation of cells in different experimental groups, determined by MTT assay at 24, 48 and 72 h after transfection. All experiments were conducted in triplicate. Statistical analysis was conducted using Student t-test for comparisons between two groups and analysis of variance for comparisons among three groups. *P<0.05 and **P<0.01 vs. control/NC group; #P<0.05 and ##P<0.01. miR, microRNA; BTK, Bruton's tyrosine kinase; PLCγ2, phospholipase Cγ2; PCNA, proliferating cell nuclear antigen; NC, negative control.