Figure 3.

Mutation in GAPDH 3′UTR results in the creation of a miR-125b binding site. (A) Sequence alignment of miR-125b binding site with wild-type (WT) or mutant (MUT) GAPDH 3′UTR. (B) Schematic structure and the free energy of potential binding between miR-125b and WT or MUT GAPDH 3′UTR. (C) miR-125b mimics or scramble NC were co-transfected with luciferase reporter constructs containing WT or MUT GAPDH 3′UTR in 293T cells and relative luciferase activity was measured. GAPDH, glyceraldehyde 3-phosphate dehydrogenase; UTR, untranslated region; NC, negative control; miR, microRNA; mfe, minimum free energy.