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. 2020 May 12;20(2):938–944. doi: 10.3892/etm.2020.8735

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Copyright: © Wang et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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ERK1/2 inhibitor PD98059 reverses the effects of Hcy on inducing Cyt c cytoplasmic translocation and apoptosis in H/R H9C2 cells. (A) Western blotting was performed to determine the expression levels of ERK and apoptosis-related proteins. Bar graphs show semiquantitative analysis of the levels of p/total ERK, p-p38, PPARγ, p53, cleaved caspase 3 and Cyt c, as determined by band density analysis. (B) The apoptosis rate was evaluated using Annexin V-FITC and PI staining, using an imaging flow cytometer. ^*P<0.05, ^**P<0.01, ^***P<0.005. Cyto, cytoplasmic; Cyt c, cytochrome c; Hcy, DL-homocysteine; H/R, hypoxia-reoxygenation; Mito, mitochondrial; p, phosphorylated; PI; propidium iodide; PPARγ, peroxisome proliferator activated receptor γ.