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ERK1/2 inhibitor PD98059 reverses the effects of Hcy on cellular and mitochondrial ROS production in H/R H9C2 cells. Representative images of green DCF-DA or red MitoSOX staining are shown. ROS production in the cell matrix and mitochondria in H/R H9C2 cells after Hcy treatment are calculated from the fluorescence intensities. **P<0.01 and ***P<0.005. DCF-DA, dichloro-dihydro-fluorescein diacetate; ROS, reactive oxygen species; Hcy, DL-homocysteine.
