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. 2020 Jun 5;20(2):1397–1404. doi: 10.3892/etm.2020.8843

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Copyright: © Zhao et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Lentivirus-mediated shRNA effectively decreases CacyBP/SIP expression in Saos-2 cells. (A) The levels of CacyBP/SIP expression in a variety of osteosarcoma cell lines. After 3 days of CacyBP/SIP lentiviral transfection, the transfection efficiency was (B) observed with fluorescence and (C) determined with reverse transcription-quantitative PCR. Magnification, x100. (D) Decreased CacyBP/SIP protein level was detected in CacyBP/SIP-shRNA lentivirus-transfected Saos-2 cells. ^**P<0.01. sh, short hairpin RNA; CacyBP/SIP, calcyclin-binding protein/Siah-1-interacting protein; Ctrl, control.