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. 2020 Jun 5;20(2):1397–1404. doi: 10.3892/etm.2020.8843

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Copyright: © Zhao et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Knockdown of CacyBP/SIP inhibits Saos-2 cell proliferation and colony formation in vitro. Saos-2 cells expressing either CacyBP/SIP-shRNA lentivirus or Ctrl lentivirus were seeded in 96-well and 6-well plates and cell growth was monitored by a Celigo Imaging Cytometer daily for 5 days. The (A) total number of cells and (B) cell growth rate were determined. Magnification, x40. (C) MTT assay and (D) colony formation assay was performed to evaluate cell proliferation. Magnification, x100. (E) Statistical analysis of colony numbers between two groups. ^**P<0.01. CacyBP/SIP, calcyclin-binding protein/Siah-1-interacting protein; sh, short hairpin RNA; Ctrl, control.