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. 2020 Jul 9;44(3):1049–1063. doi: 10.3892/or.2020.7683

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Copyright: © Pan et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 4. — Effects of shikonin on the migration and epithelial-mesenchymal transition (EMT) of A549 and H1299 cells induced by IL-6 and TNF-α. (A) Transwell chamber migration assay. After treatment with shikonin and IL-6 (50 ng/ml) or TNF-α (20 ng/ml) for 24 h, A549 and H1299 cell suspensions were subjected to a Transwell chamber migration assay. (B) Western blot and (C) real-time PCR assays were used to detect the protein and mRNA levels of E-cadherin, N-cadherin, vimentin and Snail in A549 and H1299 cells after treatment with shikonin and IL-6 (50 ng/ml) for 24 h. (D) Western blot and (E) real-time PCR assays were used to detect the protein and mRNA levels of E-cadherin, N-cadherin, vimentin and Snail in A549 and H1299 cells after treatment with shikonin and TNF-α (20 ng/ml) for 24 h. β-actin was used as an internal control. Data are shown as the mean ± SD of three independent experiments. *P<0.05, **P<0.01, compared with the IL-6- or TNF-α-treated group. IL-6, interleukin-6; TNF-α, tumor necrosis factor α.

Figure 4. — Effects of shikonin on the migration and epithelial-mesenchymal transition (EMT) of A549 and H1299 cells induced by IL-6 and TNF-α. (A) Transwell chamber migration assay. After treatment with shikonin and IL-6 (50 ng/ml) or TNF-α (20 ng/ml) for 24 h, A549 and H1299 cell suspensions were subjected to a Transwell chamber migration assay. (B) Western blot and (C) real-time PCR assays were used to detect the protein and mRNA levels of E-cadherin, N-cadherin, vimentin and Snail in A549 and H1299 cells after treatment with shikonin and IL-6 (50 ng/ml) for 24 h. (D) Western blot and (E) real-time PCR assays were used to detect the protein and mRNA levels of E-cadherin, N-cadherin, vimentin and Snail in A549 and H1299 cells after treatment with shikonin and TNF-α (20 ng/ml) for 24 h. β-actin was used as an internal control. Data are shown as the mean ± SD of three independent experiments. *P<0.05, **P<0.01, compared with the IL-6- or TNF-α-treated group. IL-6, interleukin-6; TNF-α, tumor necrosis factor α.