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. 2020 Jun 4;20(2):1775–1781. doi: 10.3892/etm.2020.8839

Figure 3.

Figure 3

Vitamin D3 treatment attenuates the increased expression levels of MMP-3, MMP-13, ADAMTS-4 and ADAMTS-5 induced by TNF-α. Reverse transcription-quantitative PCR was used to analyze the expression levels of (A) MMP-3, (B) MMP-13, (C) ADAMTS-4 and (D) ADAMTS-5. TNF-α treatment significantly increased the mRNA expression levels of MMP-3, MMP-13, ADAMTS-4 and ADAMTS-5, while vitamin D3 and PNU-74654 co-treatment significantly inhibited the expression levels of these mRNAs in chondrocytes induced with TNF-α. (E) Western blotting was used to determine the protein expression levels of MMP-3, MMP-13, ADAMTS-4 and ADAMTS-5. TNF-α treatment increased MMP-3, MMP-13, ADAMTS-4 and ADAMTS-5 expression at the protein level. Vitamin D3 and PNU-74654 inhibited the expression of MMP-3, MMP-13, ADAMTS-4 and ADAMTS-5 induced by TNF-α at the protein level. **P<0.01. MMP, matrix metalloproteinase; ADAMTS, A disintegrin and metalloproteinase with thrombospondin motifs; TNF-α, tumor necrosis factor-α; NS, non-significant.