Table I.
Gene | Primer sequence (5'-3') |
---|---|
GAPDH | F: GGAATCCACTGGCGTCTTCA |
R: GGTTCACGCCCATCACAAAC | |
Aggrecan | F: TAAACCCGGTGTGAGAACCG |
R: CCTGGGTGACAATCCAGTCC | |
Collagen II | F: CCCCTGCAGTACATGCGG |
R: CTCGACGTCATGCTGTCTCAAG | |
MMP-3 | F: GCATTGGCTGAGTGAAAGAGAC |
R: ATGATGAACGATGGACAGATGA | |
MMP-13 | F: CAGTTGACAGGCTCCGAGAA |
R: CGTGTGCCAGAAGACCAGAA | |
ADAMTS-4 | F: GCCAGCAACCGAGGTCCCATA |
R: CCACCACCAGTGTCTCCACGAAT | |
ADAMTS-5 | F: GACAAGAGTCTGGAJGGTGAGCAA |
R: GCTGCATCGTAGTGCTCCTCAT |
MMP, matrix metalloproteinase; ADAMTS, A disintegrin and metalloproteinase with thrombospondin motifs; F, forward; R, reverse.