Skip to main content
. 2020 Mar 20;9(1):55–66. doi: 10.1093/toxres/tfaa003

Figure 5.

Figure 5

Calcitriol decreased the fibroblast cell viability, proliferation, myofibroblast transdifferentation, and matrix expression through Nrf2 signaling pathway. (A) HFLIII and THP-1 cells were pre-transfected with Ctrl siRNA, Nrf2 siRNA, or VDR siRNA for 24 h and then co-cultured with the indicated treatment for another 48 h. HFLIII cells were harvested, and cell viability was measured by MTT assay. (B) The effects of Nrf2 siRNA and VDR siRNA were determined by immunoblot analysis. (C) HFLIII cells were harvested after 24 h with the same treatment above. Ki67 immunofluorescence images were captured (scale bar: 100 μm). (D) The same batch of the HFLIII cells was harvested, and the lysates were subjected to immunoblot analysis with FN, collagen IV, α-SMA, and GAPDH antibodies. The intensity of the blots from three independent experiments were quantified (right panel). Results are expressed as mean ± SD (*P < 0.05, Ctrl vs. treatment groups; #P < 0.05, P vs. calcitriol + P).