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. 2020 Jun 24;44(3):849–862. doi: 10.3892/or.2020.7659

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Copyright: © Wang et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 6. — Effect of E2F7 on miR-30c function. According to the results from EdU assay, miR-30c knockdown significantly promoted EdU uptake in (A) DU145 and (B) PC3 cell lines, which was reversed by co-transfection with E2F7 siRNA. Western blotting experiments showed that upregulation and downregulation of miR-30c resulted in the downregulation and upregulation of E2F7 expression in (C) DU145 and (D) PC3 cell lines, respectively. The values represent the mean ± SD from three experimental repeats of (E) DU145 and (F) PC3 cell lines. *P<0.05, **P<0.01 and ***P<0.0001. Scale bar, 100 µm. EdU, 5-Ethynyl-2′-deoxyuridine; miR, microRNA; miR-30c, miR-30c mimics; miR-30c i, miR-30c inhibitor; siE2F7, E2F7 siRNA.