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. 2020 Jun 5;20(2):1693–1701. doi: 10.3892/etm.2020.8841

Figure 3.

Figure 3

MIR4435-2HG OE promoted the growth of IL-1β-treated CHON-001 cells. Cells were treated with empty vector (control), IL-1β, MIR4435-2HG OE vector or IL-1β+ MIR4435-2HG OE. (A) Expression of MIR4435-2HG in CHON-001 cells was detected by reverse transcription-quantitative PCR. (B) OD values were measured in CHON-001 cells after0, 24 and 48 h using Cell Counting Kit-8 assay. (C and D) Proliferation of CHON-001 cells was assessed by Ki-67 staining. Red immunofluorescence indicated Ki-67. Blue immunofluorescence indicated DAPI. (E and F) CHON-001 cells were doubled stained with Annexin V and PI. Cell apoptosis was measured by flow cytometry. **P<0.01 vs. control; ##P<0.01 vs. IL-1β. OE, overexpression; IL, interleukin; PI, propidium iodide; OD, optical density.