Fig. 2.

The proportion of β7 positive T-cells is higher in livers of long-term PSC-IBD patients compared to short-term PSC-IBD livers and other liver diseases.

A: In situ hybridization (ISH) and immunofluorescent (IF) staining of ​ITGB7 ​and CD3 respectively on FFPE sections, 40x magnification. Representative pictures of PSC-IBD liver (upper panel) and control liver (middle). Ileum tissue was used as a positive control (lower panel). B: Fraction of CD3⁺ ​T-cells that show mRNA expression of ​ITGB7 ​in control liver (n ​= ​6), PSC-IBD_ST ​liver (n ​= ​15), PSC-IBD_LT ​liver (n ​= ​6), HCV liver (n ​= ​7) and PBC liver (n ​= ​7) using combined ISH and IF. Statistical testing was performed using Kruskal Wallis with Dunn’s correction for multiple testing. Data presented as median. A p-value <0.05 was considered statistically significant (∗p ​< ​0.05, ∗∗P ​< ​0.01).