Table I.
Antibodies used for immunostaining and flow cytometry.
| Antibody (clone) | Manufacturer (cat. no.) | Utilization | Dilution |
|---|---|---|---|
| Tyrosine hydroxylase (TH) | Abcam (ab112) | IF | 1:1,000 |
| CD8-PE (53-6.7) | eBioscience; Thermo Fisher Scientific, Inc. (12-0081-82) | IF, FC | 1:100 |
| CD4-PE (RM4-5) | eBioscience; Thermo Fisher Scientific, Inc. (12-0042-82) | IF, FC | 1:100 |
| CD45-APC (30-F11) | eBioscience; Thermo Fisher Scientific, Inc. (17-0451-82) | FC | 1:100 |
| CD31-PE (390) | eBioscience; Thermo Fisher Scientific, Inc. (12-0311-82) | FC | 1:100 |
| ICAM1-FITC (YN1/1.7.4) | eBioscience; Thermo Fisher Scientific, Inc. (11-0541-82) | FC | 1:100 |
| CD3-PE (145-2C11) | eBioscience; Thermo Fisher Scientific, Inc. (12-0031-82) | FC | 1:100 |
| LFA1-FITC (M18/2) | eBioscience; Thermo Fisher Scientific, Inc. (11-0181-82) | FC | 1:100 |
PE, phycoerythrin; APC, allophycocyanin; ICAM1, intercellular adhesion molecule 1; LFA1, lymphocyte function-associated antigen-1; IF, immunofluorescence; FC, flow cytometry.