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. 2019 May 28;1:100002. doi: 10.1016/j.jtauto.2019.100002

Fig. 4.

Fig. 4

Expression of TLR 2 and TLR 4 on human spermatozoa surface.(a) The fluorescence intensity of TLR 2 and TLR 4 was represented in the spermatozoa of fertile donor (control) and infertile LCS/BS patient sample. Both TLR 2 and TLR 4 are stained green with FITC staining. Nucleus is stained blue with Hoechst 33324. Merged panel represented both TLR 2/4 and nuclear staining at the same time. Image J was used to measure mean fluorescence intensity for TLR 2 and TLR 4. Corrected intensities are calculated for control and LCS/BS (n ​= ​5). Statistical analysis is conducted after normalization to the number of Hoechst stained cells. The IF data showed significant increases in (b) TLR 2 (*p ​< ​0.0037) and (c) TLR 4 (**p ​< ​0.0002) in LCS/BS samples compared with control samples. Experiment was repeated thrice. LCS/BS: Leukocytospermia associated with bacteriospermia; IF: immunofluorescence.