Figure 2.

TRIM31 confers TMZ resistance in glioma cells in vitro. (A) mRNA and protein expression levels of TRIM31 in cells transfected with empty plasmid, TRIM31 overexpression plasmid, siTRIM31 and siNC. (B) Cell viability of A172 and U251 cells transfected with the indicated plasmids. (C) IC₅₀ values of TMZ in A172 and U251 cells transfected with the empty plasmid and TRIM31 overexpression plasmid. (D) Colony formation in A172 and U251 cells transfected with empty plasmid and TRIM31 overexpression plasmid and treated with 100 µM TMZ. ^*P<0.05, ^**P<0.01, ^***P<0.001. Data are presented as the mean ± standard deviation of three independent experiments. TMZ, temozolomide; si, small interfering; NC, negative control; TRIM31, tripartite motif-containing 31.