Figure 3.

TRIM31 decreased TMZ-induced glioma cell apoptosis. (A) Apoptotic rates of A172 and U251 cells transfected with empty plasmid and TRIM31 overexpression plasmid after 48 h of TMZ treatment (100 µM). (B) Apoptotic rate of U251 cells transfected with siTRIM31 and negative control after 48 h of TMZ treatment (100 µM). (C) Protein expression levels of p53 were analyzed by western blot in U251 and A172 cells transfected with TRIM31 plasmids and treated with TMZ. ^**P<0.01, ^***P<0.001. Data are presented as the mean ± standard deviation of three independent experiments. Si, small interfering; TMZ, temozolomide; TRIM31, tripartite motif-containing 31.