Fig. 4.

Sex-dependent and independent effects of IFNAR1 deficiency on RNA expression of viral HIVgp120 and host genes. The mRNA expression in the hippocampus of 11-14 months-old mice, were detected by quantitative RT-PCR as described in the material and method section. HIVgp120 (a); CCL2 (b) and CCL5 (c), CCL3 and CCL4 (d); CCR5 and CXCR4 (e); CXCL12 and MX1 (f); CXCL10 and CXCL11 (g). The obtained CT values were normalized to the level of GAPDH mRNA. Genotypes: WT (WT), HIVgp120tg (GP), IFNAR1KO (KO), and IFNAR1KO × gp120 (KOGP). Values are presented in combined box-dot plots with the 25th and 75th percentiles. The middle line of the box shows the median, and the mean is indicated by a “+”; ****P < 0.0001, ***P < 0.001, **P < 0.01, *P < 0.05; ANOVA and Tukey’s HSD post hoc test; n = 3 per sex/experimental group/genotype or 6 animals (3 males and 3 females combined) per group/genotype (total n = 24 animals); n.s., not significant