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. 2020 Jul 13;44(3):1037–1048. doi: 10.3892/or.2020.7686

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Copyright: © Jeddo et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 2. — MELK inhibition is associated with suppression of proliferation in vitro. (A and B) MELK expression was knocked down using si-MELK, and the efficiency of knock down was determined using western blotting and PCR in MNNG/HOS cells. (C) A CCK-8 assay revealed that proliferation was reduced following knockdown of MELK. (D) A cell viability assay was used to determine the IC₅₀ of OTSSP167, which was determined to be 40 nM. (E) Western blots revealing the concentration-dependent inhibition of MELK by OTSSP167. (F) A CCK-8 proliferation assay revealed OTSSP167 inhibited proliferation of osteosarcoma cells compared with the vehicle control. **P<0.01, ***P<0.001. MELK, maternal embryonic leucine zipper kinase; si, small interfering; CCK-8, CELL Counting Kit-8.