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. 2020 Jun 16;44(3):927–938. doi: 10.3892/or.2020.7647

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Copyright: © Zhou et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 3. — Downstream effect of ANGPTL4 following binding to integrin α5β1 on surface of the tumor cell membrane. (A) Co-IP was performed and the immunoprecipitates were analyzed using western blot analysis with antibodies against integrin α5β1, claudin-5, ANGPTL4 and GAPDH. (B) Statistical results of the co-IP assay. The data are presented as the mean ± SD. **P<0.01 vs. SKOV3. (C and D) The regulatory effect following silencing of integrin α5β1 on the ANGPTL4-induced resistance of SKOV3 cells in vitro. The data are presented as the mean ± SD. **P<0.01 vs. SKOV3^shMock/CM/carboplatin or SKOV3^shMock/adipocyte/carboplatin). (E and F) The regulatory effect following silencing of claudin 5 on the ANGPTL4-induced resistance of SKOV3 cells in vitro. The data are presented as the mean ± SD. **P<0.01 vs. SKOV3^shMock/CM/carboplatin or SKOV3^shMock/adipocyte/carboplatin). ANGPTL4, angiopoietin-like 4; Co-IP, co-iummunoprecipitation; sh, short hairpin; CM, conditioned medium.