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. 2020 Jun 12;20(2):1653–1663. doi: 10.3892/etm.2020.8887

Figure 2.

Figure 2

miR-17-5p decreases IL-1β-induced cell apoptosis and ECM degradation in chondrocytes. Chondrocytes were transfected with miR-17-5p mimic, miR-NC, in-miR-17-5p or in-miR-NC prior to IL-1β treatment. (A and B) The expression of miR-17-5p was measured by reverse transcription-quantitative PCR in chondrocytes transfected with (A) the miR-17-5p mimics and (B) in-miR-17-5p. (C and D) The proportion of apoptotic chondrocytes was determined by flow cytometry following transfection with (C) the miR-17-5p mimics and (D) in-miR-17-5p. (E and F) The protein expression levels of ECM-associated proteins Collagen II, Aggrecan and MMP13 were determined by western blotting in chondrocytes transfected with (E) the miR-17-5p mimics and (D) in-miR-17-5p. *P<0.05. miR, microRNA; IL-1β, interleukin-1β; ECM, extracellular matrix; NC, negative control; in, inhibitor; MMP13, matrix metalloproteinase 13; PI, propidium iodide.