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. 2020 Jun 12;20(2):1653–1663. doi: 10.3892/etm.2020.8887

Figure 3.

Figure 3

EZH2 is a target of miR-17-5p in chondrocytes. (A) The predicted binding sites of miR-17-5p and EZH2 3'UTR. (B and C) The luciferase activity in chondrocytes co-transfected with EZH2#1 3'UTR-WT or EZH2#1 3'UTR-MUT and (B) miR-NC or the miR-17-5p mimic, or (C) in-miR-NC or in-miR-17-5p was determined using a dual-luciferase reporter assay. (D) The RIP assay was used to validate the relationship between miR-17-5p and EZH2. (E) EZH2 protein expression was measured in chondrocytes transfected with miR-NC, miR-17-5p mimic, in-miR-NC or in-miR-17-5p. *P<0.05 vs. NC. EZH2, enhancer of zeste homolog 2; miR, microRNA; 3'UTR, 3'-untranslated region; WT, wild-type; MUT, mutant; NC, negative control; in, inhibitor; Ago2, argonaute RISC catalytic component 2; IgG, immunoglobulin G.