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. 2020 Jun 12;20(2):1653–1663. doi: 10.3892/etm.2020.8887

Figure 4.

Figure 4

EZH2 facilitates IL-1β-induced chondrocyte apoptosis and ECM degradation. Chondrocytes were transfected with EZH2 overexpression vector, pcDNA, si-EZH2 or si-NC prior to IL-1β stimulation. (A and B) The protein expression of EZH2 was measured by western blotting in chondrocytes transfected with (A) the EZH2 overexpression vector and (B) si-EZH2. (C and D) The proportion of apoptotic cells was assessed by flow cytometry in chondrocytes transfected with (C) the EZH2 overexpression vector and (D) si-EZH2. (E and F) The protein expression levels of extracellular matrix-associated proteins Collagen II, Aggrecan and MMP13 were detected by western blotting in chondrocytes transfected with (E) the EZH2 overexpression vector and (F) si-EZH2. *P<0.05. EZH2, enhancer of zeste homolog 2; IL-1β, interleukin-1β; si, small interfering RNA; NC, negative control; MMP13, matrix metalloproteinase 13; PI, propidium iodide.