Figure 5.

ROS levels of H9C2 cells determined by DCF-DA staining. shRNA- PPAR-γ-1 was transfected into H9C2 cells treated with Catalpol (0 µM) and DOX (1 µM), and incubated for 24 h. Subsequently, ROS levels were detected by DCF-DA staining (magnification, x400). ^**P<0.01 and ^***P<0.001 vs. control; ^###P<0.001 vs. DOX (1 µM); ^ΔΔΔP<0.001 vs. catalpol (20 µM) + DOX (1 µM) + shRNA-NC. ROS, reactive oxygen species; shRNA, short hairpin RNA; DOX, doxorubicin; NC, negative control; PPAR-γ, peroxisome proliferator-activated receptor-γ.