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Anti-proliferative effects of neferine in ESCC cell lines. (A) Neferine structure. (B and C) KYSE30, KYSE150 and KYSE510 cells were treated with neferine (0, 10 and 15 µM) for (B) 24 h and (C) 48 h. Cell viability was detected by the CCK-8 assay. (D and E) Results of colony formation assays in KYSE30 and KYSE150 cells incubated with neferine (0, 10 and 15 µM). *P<0.05 vs. 0 µM of neferine or vs. the control. ESCC, esophageal squamous cell carcinoma; CCK-8, Cell Counting Kit-8.
