Neural Induction Media (NIM)
| Ingredient | Volume | Final concentration |
|---|---|---|
| DMEM | 250 mL | ------- |
| F12 | 250 mL | |
| Non-essential amino acids | 5 mL | 1% |
| Heparin | 50 μL | 2 μg/mL |
| N2 (100x) (added fresh) | 5 mL | 1% |
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